TransStart KD Plus DNA Polymerase
TransStart® KD Plus DNA Polymerase is a genetically modified high fidelity DNA polymerase. This enzyme provides higher amplification capability than traditional Pfu DNA polymerase and fast amplification speed equal to Taq DNA polymerase (1 kb/min). Due to strong 3'-5' exonuclease activity, this enzyme offers 108-fold fidelity as compared to EasyTaq® DNA Polymerase.
| Cat. No. | AP301 |
| Specification | dNTPs-free | AP301-01 | 100 units |
| AP301-02 | 200 units |
| AP301-03 | 6×200 units |
| dNTPs (2.5 mM) | AP301-11 | 100 units |
| AP301-12 | 200 units |
| AP301-13 | 6×200 units |
| Concentration: | 1 units/μl |
| Storage: | at -20°C for two years |
| Description | PCR product carries blunt end, which can be directly cloned into pEASY® -Blunt vectors. |
| Amplification of genomic DNA fragment up to 15 kb. |
| Amplification of plasmid DNA fragment up to 20 kb. |
| Advantages | Novel hot start, high specificity |
| High efficiency amplification |
| High fidelity |
| Fast speed |
| Application | Rapid amplification of high fidelity PCR, blunt end cloning, site-directed mutagenesis |
| Storage Buffer | 5 0 mM Tris-Cl (pH 8.0),50 mM KCl,1 mM DTT,0.1 mM EDTA,Stabilizers,50% glycerol
|
Kit Content
| Component | AP301-01/11 | AP301-02/12 | AP301-03/13 |
| TransStart® KD Plus DNA Polymerase | 100 U×1 | 200 U×1 | 200 U×6 |
| 5×TransStart® KD Plus Buffer | 1.2 ml ×1 | 1.2 ml ×2 | 1.2 ml ×12 |
| 2.5 mM dNTPs | - / 450 μl ×1 | - / 900 μl ×1 | - / 900 μl ×6 |
| 50 mM MgSO4 | 200 μl×1 | 400 μl×1 | 1 ml ×1 |
| 6×DNA Loading Buffer | 500 μl×1 | 1 ml ×1 | 1 ml ×2 |
Reaction Components
| Component | Volume | Final Concentration |
| Template | Variable | as required |
| Forward Primer (10 μM) | 1-2 μl | 0.2-0.4 μM |
| Reverse Primer (10 μM) | 1-2 μl | 0.2-0.4 μM |
| 5×TransStart® KD Plus Buffer | 10 μl | 1× |
| 2.5 mM dNTPs | 4 μl | 0.2 mM |
| TransStart® KD Plus DNA Polymerase | 1 μl | 2.5 units |
| ddH2O | Variable | - |
| Total volume | 50 μl | -
|
TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research.
In March 21, 2006, TransGen Biotech was incorporated in Beijing, China. The company's headquarter, R&D, and manufacturing facility are located in Beijing. To date, the company has more than 100 biologists in Beijing, and has more than 30 distribution centers covering all major cities in China. Our extensive R&D experience and state-of-the-art facilities enable us to keep generating the most innovative and the highest quality products. In 2006, 2009 and 2012, the company was consecutively awarded as one of the "High Tech Corporation in Beijing" by Beijing local government.
Currently, our products cover: Plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
As the leading bioreagent company in China, we are looking forward to partner with you in your quest for ground-breaking life science discovery.
