TransTaq-T PCR SuperMix
TransTaq®-T PCR SuperMix is a ready-to-use mixture of TransTaq®-T DNA Polymerase, dNTPs, and optimized buffer. This SuperMix is provided at 2× concentration and used at 1× concentration by adding template, primers and H2O. Efficiency of PCR products with "A" is equal to EasyTaq® DNA polymerase. It is more suitable for high fidelity TA cloning.
Cat. No. |
AS122 |
Specification | Mix(-dye) | AS122-01 | 1 ml |
| AS122-02 | 5×1 ml |
| Mix(+dye) | AS122-11 | 1 ml |
| AS122-12 | 5×1 ml |
| Storage: | at -20°C for two years |
| Description | Fidelity is 18 times higher than EasyTaq® DNA Polymerase. |
| The extension rate is about 1-2 kb/min. |
| Template-independent "A" can be generated at the 3' end of the PCR product. PCR products can be cloned into pEASY®-T vectors. |
| Amplification of genomic DNA fragment up to 8 kb. |
| Advantages | High efficiency amplification |
| Excellent stable performance |
| Applications | Complex template |
| TA cloning |
Reaction Components
| Component | Volume | Final Concentration |
| Template | Variable | as required |
| Forward Primer (10 μM) | 1-2 μl | 0.2-0.4 μM |
| Reverse Primer (10 μM) | 1-2 μl | 0.2-0.4 μM |
| 2× TransTaq®-T PCR SuperMix | 25 μl | 1× |
| ddH2O | Variable | - |
| Total volume | 50 μl | - |
Notes
Completely thaw the contents in the tube and mix well before each use.
TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research.
In March 21, 2006, TransGen Biotech was incorporated in Beijing, China. The company's headquarter, R&D, and manufacturing facility are located in Beijing. To date, the company has more than 100 biologists in Beijing, and has more than 30 distribution centers covering all major cities in China. Our extensive R&D experience and state-of-the-art facilities enable us to keep generating the most innovative and the highest quality products. In 2006, 2009 and 2012, the company was consecutively awarded as one of the "High Tech Corporation in Beijing" by Beijing local government.
Currently, our products cover: Plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
As the leading bioreagent company in China, we are looking forward to partner with you in your quest for ground-breaking life science discovery.
