Use of HA-350 can bring the following benefits for ethanol prodution:
1.High stability with low pH;
2.Decrease viscosity quickly;
3.Decrease formation of non-fermenting sugars;
4.No need of calcium ion under majority processing conditions;
5.Effective liquefaction of different starch materials, such as tapioca, corn, wheat and rice.
DESCRIPTION
HA-350 is derived from a strain of Bacillus licheniformis using submerged fermentation and refining extraction techniques. Boli HA-350 is an endoamylase, which rapidly decreases the viscosity of a gelatinous starch solution by randomly hydrolyzing starch, glycogen and its degradation products within the alpha-D-1, 4 glucosidic bonds to produce soluble dextrins and oligosaccharides.
APPLICATION
HA-350 is a robust alpha-amylase suitabel for ethanol industry.
PRODUCT CHARACTERISTICS
Declared Enzyme: Alpha-Amylase
Systematic Name: 1, 4-α -D-glucan glucanohydrolase
Activity: 50, 000 U/ml (minimum)
Appearance: Light to dark brown liquid
Product pH: 5.5 to 7.0
Specific gravity: 1.15 to 1.25 g/ml
EFFECT OF pH
HA-350 is active over a wide pH range, depending on the application. For maximum activity, the optimal pH for Boli HA-350 is 5.6 to 6.2. The exact pH optimum will depend on many process variables, including temperature, substrate concentration, and time.
EFFECT OF TEMPERATURE
For interval liquefaction, the optimum temperature of？HA-350 is above 85ºC. It liquefies starch substrates promptly at 95 to 97ºC and maintains highly active at 100ºC. For consecutive liquefaction,HA-350 demonstrates exceptional thermo-stability at temperatures up to 105 to 110ºC, maintains its stability for 5 to 7 minutes and keeps on liquefying rapidly and completely.
CONCENTRATION OF CALCIUM
The presence of calcium increases the thermal stability of HA-350. This enzyme requires a very low calcium concentration and calcium additions of 20 to 50 ppm (dry solids basis) are sufficient to ensure enzyme stability.
INHIBITORS
Copper, titanium, and cobalt ions are moderate inhibitors for？HA-350. Aluminum, lead and zinc ions act as strong inhibitors.
USAGE GUIDELINES
In general, first adjust the pH to 5.6 to 6.2 and then adding？ HA-350. Calcium is not needed. After the enzyme is added, heat the slurry to liquefy at the temperature of 85 to 90ºC for 90 to 150 minutes. The final DE value should be maintained at 10 to 12.
Dosage of HA-350 is dependent on the application,？substrate concentration, pH, temperature and other processing conditions. To optimize the dosage, it is recommended to conduct a number of liquefaction trials prior to routine use of this product. A good starting point for dosage rate is 0.33 to 0.64 kg/TDS.
PACKAGING
HA-350 is packaged in 30 kg or 1150 kg plastic drums. Customized packaging is available upon request.
STORAGE
Boli HA-350 should be stored below 25° C in its original packaging, sealed and unopened, protected from the sun. This enzyme has been formulated for optimal stability. However, enzymes gradually lose activity over time. Extended storage and/or adverse conditions such as higher temperature may lead to a higher dosage requirement.
SAFETY & ENZYME HANDLING
Inhalation of enzyme dust and mists should be avoided. In case of contact with the skin or eyes, promptly rinse with water for at least 15 minutes. For detailed handling information, please refer to the appropriate Material Safety Data Sheet.
KOSHER CERTIFICATE
We are proud of our state-of-the-art factory Jiangsu Boli Bioproducts Co., Ltd. (hereinafter refer to as "Boli Bioproducts") as a professional manufacturer of quality and cost-effective enzymes that bring added-value to our customers. With more than twenty years expertise in enzyme research and development, Boli Bioproducts has been recognized by its customers as a reliable partner that helps to improve their bottom-line because of its consistent high quality, most competitive price and unparalleled excellent services.
Our enzyme products can be divided into industrial grade and food grade, and are available in both liquid and solid (powder) forms. Among various applications for these enzymes are: fuel ethanol production, grain processing, food baking, brewing, textiles and animal feed. Our top products such as Glucoamylase, Alpha-Amylase, Beta-Glucanase, Catalsae and Protease are widely accepted and highly appreciated by our customers because of the benefits --- higher enzyme activity, superior pH tolerance and thermal stability, greater starch-ethanol conversion rate, less enzyme dosage, less emission of waste, easier processing, more output --- that are greatly helpful to improve their bottom line.
What makes us different?
As one of the leading enzyme manufacturers in China, we excel ourselves as a long-term business partner that brings added-value to our customers with the following advantages:
Technical advantages:
Our strong R & D team has more than two decades of expertise in enzyme research and development. We are in close corporations with the prestigious research institutes, universities and academies in China. Appointed as one of the participating companies to tackle key problems of scientific and technological difficulties for the National High-Tech R & D Program of China's Ministry of Science and Technology, Boli Bioproducts has become the industrialization experimental site of Southern Yangtze University's School of Biotechnology. As a result of its dedication to research and development, Boli Bioproducts has been very successful in developing and patenting several new enzymes in China. Boli Bioproducts has also been awarded the High & New Technology Enterprise of China.
Quality advantages:
The robust enzyme activities and the superior stabilities are two of the major advantages of our products comparing to others available in market. This means the use of our products results in more output and less cost. Another advantage worth to mention is the adaptability to low pH environment that our product Thermostable Alpha-Amylase (Low pH) Boli HT can offer, which leads to easy processing and thus reduces processing cost.
Service advantages:
Our dedicated technical service team are always happy to answer any of your questions regarding our products in a professional way on 24/7 basis.
Price advantages:
All of our products are competitively priced. Considering the high quality and reliable service we offer, the use of our products creates added-value surpassing your expectation.
Our qualification
ISO9001 Quality Management System Certified;
ISO14001 Environmental Management System Certified;
ISO22000 Food Safety Management System Certified;
Kosher Certified;
Halal Certified;
Manufacturing License for food additives in China;
Awarded the High & New Technology Enterprise of China;
Recognized as Environmental-friendly Enterprise by local government
We fully understand that we would never be where we are today if without the valuable support from YOU, our customers. Commitment, Integrity, Strive for Customer Satisfaction, that is what you can always expect from us as your reliable business partner that catalyzes your business success.
