Product Specifications
| Markets: |
North America,South America,Eastern Europe,Southeast Asia,Africa,Oceania,Mid East,Eastern Asia,Western Europe |
| Classification: |
Protein |
Product Description
INDUMY nuclease is a recombinant endonuclease expressed in E. Coli and purified to homogeneity. This enzyme attacks and degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) and is effective over a wide range of conditions. The enzyme completely digests nucleic acids to 5'-monophosphate terminated oligonucleotides 2 to 5 bases in length which is below the hybridization limit. NDUMY nuclease preparations possess exceptionally high specific activities and are supplied free from measurable protease activities and viral contaminants.
This enzyme is ideal for a wide variety of applications where complete digestion of nucleic acids is desirable.
1, Viscosity reduction in protein extracts
2, Sample preparation for 2D gel electrophoresis
3, Removal of nucleic acid contaminants from recombinant protein preparations
This enzyme is designed with (His)6 tag and so it can be easily removed during the downstream procedures. It is functional between pH 6 and 10 and from 0-42C and requires 1-2 mM Mg2+ for activation. The enzyme is also active in the presence of ionic and non-ionic detergents, reducing agents, The product is supplied as a 0.2 μ M filtered solution in 50% glycerol.
Unit definition
One unit of INDUMY™ Nuclease is defined as the amount of enzyme that causes a A260 of 1.0 in 30 min, which corresponds to complete digestion of 37 μ G of DNA. Standard reaction conditions are 1 mg/ml sonicated DNA substrate in 50 mM Tris-HCl pH 8.0, 0.1 mg/ml BSA, 1 mM MgCl2, incubated at 37C; Measured as perchloric acid-soluble digestion product.
Storage
INDUMY Nuclease is supplied in 50% glycerol containing 50 mM Tris-HCl pH 8.0, 20 mM NaCl and 2 mM MgCl2. The enzyme preparation is stable for 2 years when stored at below20C.
DO NOT store at below 70C
Hangzhou Biodoor Biotechnology Co., Ltd., is a cutting-edge drug-based corporation. We are devoted to the research, development, manufacture and trade of recombinant enzymes and polypeptides, as well as pharmaceutical raw materials and intermediates. Our dedicated, motivated and talented staff is responsible for developing protein isolation and purification process and polypeptide synthesis.
So Junfeng also provides technical services in these areas. Our products include recombinant functional enymes as penicillin acylase, celluase, catase, lipase as well as enzymes using in biotechnology area. Recombinant Enterokinase and b-lactanase are now available in our company.
