Product Specifications
| Prod Model: |
AI101 |
| Markets: |
North America,South America,Eastern Europe,Southeast Asia,Africa,Oceania,Mid East,Eastern Asia,Western Europe |
| Appearance: |
Solid |
| Classification: |
Ribonuclease Inhibitor |
| Function: |
Ribonuclease Inhibitor |
Product Description
Ribonuclease Inhibitor
RNase Inhibitor is a recombinant protein isolated from E. coli strain carrying human placenta ribonuclease gene. It specifically inhibits RNase A, RNase B, RNase C and forms 1:1 complex with RNase. It is unable to inhibit RNase 1, RNase T1, S1 nuclease RNase H and Aspergillus-originated RNase. It shows no inhibitory effect on DNA Polymerases, AMV, M-MLV, SP6,T7 and T3 RNA Polymerases.
Cat. No. |
AI101 |
Specification |
AI101-01 |
2000 units |
| AI101-02 | 5×2000 units |
| Concentration: |
50 units/μl |
Storage: |
at -20°C for one year |
Storage Buffers |
50 mM Tris-HCl (pH 7.5), 0.1 mM EDTA, 10 mM DTT, 100 mM NaCl, 50% glycerol |
Unit Definition |
One unit (U) is defined as the amount of the polymerase required for inhibiting 50% of 5 ng RNaseA activity. |
Applications |
cDNA synthesis, polysome extraction, in vitro translation, in vitro cell-free transcription, in vitro transcription of SP6 or T7 RNA polymerase. |
Notes |
? RNase Inhibitor shows highest activity within pH 7.0-pH 8.0, a wider pH range can inhibit its activity. |
? Foaming or vigorous vortex can result in the inactivation of RNase Inhibitor. |
Component | Volume |
| Total RNA/mRNA | 50 ng-5 μg/5-500 ng |
| Anchored Oligo(dT)18 Primer (0.5 μg/μl) | 1 μl |
| or Random Primer (0.1 μg/μl) | 1 μl |
| or GSP | 2 pmol |
| 10 mM dNTPs | 1 μl |
| 5× RT Buffer | 4 μl |
| Ribonuclease Inhibitor | 0.5 μl |
| EasyScript®/TransScript® RT | 1 μl |
| For anchored oligo(dT)18 primer or GSP, incubate at 42oC for 30 minutes. |
| For random primer, incubate at 25oC for 10 minutes, then at 42oC for 30 minutes |
| Incubate at 85oC for 5 minutes to inactive EasyScript®/TransScript® RT. |
TransGen Biotech, Inc. is a researcher, developer, manufacturer and distributor of more than 200 molecular and cellular biology products and kits for life science research and molecular diagnostics. In 2001, the company was founded by three scientists with a mission to produce innovative and cost-effective products for life science research.
In March 21, 2006, TransGen Biotech was incorporated in Beijing, China. The company's headquarter, R&D, and manufacturing facility are located in Beijing. To date, the company has more than 100 biologists in Beijing, and has more than 30 distribution centers covering all major cities in China. Our extensive R&D experience and state-of-the-art facilities enable us to keep generating the most innovative and the highest quality products. In 2006, 2009 and 2012, the company was consecutively awarded as one of the "High Tech Corporation in Beijing" by Beijing local government.
Currently, our products cover: Plasmid based DNA markers, high efficiency chemically competent cells, 5 minutes PCR product cloning and expression vectors, a variety of PCR enzymes and supermix, RNase H deficient and high temperature RT enzymes, qPCR and qRT-PCR supermix, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, unstained and prestained protein markers, western blot markers, and protein purification resins, cell culture and transfection reagents, antibodies.
As the leading bioreagent company in China, we are looking forward to partner with you in your quest for ground-breaking life science discovery.
