Classification: Chemical Auxiliary Agent
CAS No.: 9028-56-2
Other Names: 3alpha-Hydroxysteroid: NAD(P)+ oxid...
Place of Origin: Shanghai, China (Mainland)
Type: White powder, lyophilized
Usage: Diagnostic enzymes
Specifications
It catalyzes the dehydrogenation of hydroxysteroid. It is useful for the determination of total bile acids in clinical analysis.
3alpha-Hydroxysteroid dehydrogenase catalyzes the dehydrogenation of hydroxysteroid. It is useful for the determination of total bile acids in clinical analysis.
Product Description
Appearance: White powder, lyophilized
Source: Microorganism
Enzyme Comission Number: EC 1.1.1.50
Storage Temperature -20° C
Specific activity: ≥ 50U/mg protein
Unit definition: One unit will oxidate one micromole of androsterone per min at pH 8.9 at 25° C.
Properties
Molecular Weight: 28 kDa (SDS-PAGE)
Isoelectic point: 7.5
Michaelis constant: 3.0× 10-5M(androsterone) 6.0× 10-6M(NAD+)
Optimum pH: 10.5
Optimum temperature: 60° C
PH Stability: 4.5~10.5
Thermal Stability: < 50° C (pH7.2, 20min)
Inhibitors: Ag+, Hg2+
Preparation Instructions
The enzyme is reconstituted in 30mM potassium phosphate buffer, pH 7.2 for activity assay.
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