Biological safety cabinet can be divided into three categories: one, two and three.
A class of biological safety cabinet can protect the working personnel and the environment without protecting the sample. The principle and flow of laboratory fume hood, the difference lies in the exhaust port is provided with a HEPA filter. All types of biological safety cabinets are used in the exhaust and inlet HEPA filter. A biological safety cabinet itself wind machine, depending on the external connected in a ventilation duct fan driven air, because it is not to test products or products to provide protection, has been less used.
Two level biological safety cabinet is the most widely used cabinet type. Like the first biological safety cabinet, the second level biological safety cabinet also has the air flow into the front window openings, which is called the "air inlet flow", which is used to prevent the possible generation of aerosol in the microbial operation from the window to escape. Different from the first biological safety cabinet, the non filtered air inlet will be trapped in the wind grid before reaching the working area, so the test sample will not be polluted by the outside air. A unique feature of the class II biological safety cabinet is through HEPA filters to filter the vertical laminar flow from the top of the safety cabinet blowing, known as the "subsidence". The sinking air flow continues to blow through the safety cabinet work area, in order to protect the test product in the cabinet is not contaminated by outside dust or bacteria.
According to the provisions of the 49, the two level of biological safety cabinet in accordance with the inlet air velocity, exhaust mode and circulation can be divided into 4 levels: A1 type, A2 type (B3 type), B1 type and B2 type. All two levels of biological safety cabinets are available to provide staff, environment and product protection.
The airflow velocity of the front window of the A1 type safety cabinet should be at least 0.38m/s. 70% gas recirculation through the HEPA filter to the working area, 30% of the gas through the exhaust port filter out. The airflow velocity of the front window of the A2 type safety cabinet should be at least 0.5m/s. 70% gas recirculation through the HEPA filter to the working area, 30% of the gas through the exhaust port filter out. The design of the negative pressure surrounding the pollution area of the A2 type safety cabinet prevents the leakage of the material in the cabinet.
The two level B type biological safety cabinet is connected with the safety cabinet of the exhaust system. Connect the power supply of the exhaust pipe of the safe cabinet, and can keep the negative pressure under the power supply. The minimum or average value of the air velocity in the front window is at least 0.5m/s (100fpm). B1 type 70% gas through the exhaust port HEPA filter to exclude, 30% of the gas through the gas outlet HEPA filter to the working area. B2 type 100% full - row type safety cabinet, no internal circulation air flow, but also provide biological and chemical safety control.
Two biological safety cabinet cabinet type the performance ratio
Level three biological safety cabinet is designed for the 4 level of laboratory biological safety level, is currently the world's highest level of safety protection. Cabinet body is completely sealed, 100% discharge type, all gas can not participate in the circulation, the staff through the connection in the cabinet of the gloves to operate, commonly known as the glove box (Golve box), the test by double door transfer box and safety cabinet to ensure that it is not contaminated, for biological experiments in high risk.
A2 type two level biological safety cabinet, which can be used for the operation of the circulating chemical gas, can be used for the Collar type level biological safety cabinet. The difference between the discharge pipe cover and the ordinary hard pipe is the air inlet hole; the discharge pipe is connected with the outer drain pipe, and then a wind turbine is received. The inlet air vent on the discharge pipe cover is essential for maintaining the balance between the intake flow and the air flow of the A2 type two biological safety cabinet. If the use of sealed air pipe, inlet flow will be too strong may lead to safety cabinet of effective protection; cover and discharge pipe on the air inlet can be from indoor inhaled air, and will not affect the security cabinet airflow balance. This condition applies only to trace toxic chemicals.
If you do not allow the circulation of chemical gas, you must use the B2 - type two - level biological safety cabinet with a hard tube. As the B type safety cabinet is not an independent balance system, it can only be built in the wind turbine, safe cabinet depends on the exhaust fan to create the air inlet flow. This type of safety cabinet in the installation and maintenance will be more complex, because the exhaust fan must be kept in balance with the built-in fan, otherwise it will lead to the failure of the safety performance of the operator or product.
Shenzhen Yong Jie Cleanroom Engineering Co., Ltd. Was founded in 2007, is China's professional air purification system engineering to provide a large scale construction company. Now is the vice president of the Shenzhen clean industry association, is China's line board industry association, China's flat panel display industry association, with a two degree of purification engineering qualification. Through the accumulation of many years of experience and technology, we have been committed to engage in purification engineering design, construction and central constant temperature and humidity air conditioning engineering, send return air system, color plate compartment and purification equipment R & D, design, manufacturing, sales and service as one integrated professional purification company. Yong Jie in the bio pharmaceutical, medical device, food, cosmetic, electronics manufacturing, Photoelectric optics, LCD/LED, printing and packaging, fine chemical industry in the design and construction of the innumerable classical engineering and customer access to a high degree of recognition.
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