Product Specifications
| Prod Model: |
AP231 |
| Markets: |
North America,South America,Eastern Europe,Southeast Asia,Africa,Oceania,Mid East,Eastern Asia,Western Europe |
| Classification: |
Polymerase |
Product Description
Description
TransStart FastPfu Fly DNA Polymerase is a hot start, high fidelity and high processivity DNA Polymerase. The fidelity of TransStart® FastPfu Fly DNA Polymerase is 108 times higher than EasyTaq® DNA polymerase. TransStart® FastPfu Fly DNA Polymerase has extension rate up to 6 kb/ min. Compared with TransStart® FastPfu DNA Polymerase, TransStart® FastPfu Fly DNA Polymerase has faster extension rate, higher fidelity, and higher amplification efficiency.
1. Fidelity is 108 times higher than EasyTaq® DNA Polymerase.
2. The extension rate is about 2-6 kb/min.
3. PCR products can be directly cloned into pEASY® -Blunt vectors.
4. Amplification of genomic DNA fragment up to 15 kb.
5. Amplification of plasmid DNA fragment up to 20 kb.
Advantages
1. Novel hot start
2. Rapid amplification
3. High fidelity
4. Powerful amplification capability
5. High sensitivity
6. High yield
Applications
1. Complex templates
2. High fidelity PCR
3. High yield and fast PCR
4. Blunt end cloning
5. Site-directed mutagenesis
dNTPs-free |
AP231-01 |
250 units |
AP231-02 |
500 units |
AP231-03 |
6×500 units |
dNTPs
(2.5 mM) |
AP231-11 |
250 units |
AP231-12 |
500 units |
AP231-13 |
6×500 units |
Kit Content
| Component | AP231-01/11 | AP231-02/12 | AP231-03/13 |
| TransStart ® FastPfu Fly DNA Polymerase | 250 U×1 | 500 U×1 | 500 U×6 |
| 5× TransStart ® FastPfu Fly Buffer | 1.2 ml ×1 | 1.2 ml ×2 | 1.2 ml ×12 |
| 2.5 mM dNTPs | - / 450 μl ×1 | - / 900 μl ×1 | - / 900 μl ×6 |
| 50 mM MgSO4 | 200 μl×1 | 400 μl×1 | 1 ml ×1 |
| PCR Stimulant | 200 μl×1 | 400 μl×1 | 1 ml ×1 |
| 6×DNA Loading Buffer | 500 μl×1 | 1 ml ×1 | 1 ml ×2 |
Beijing TransGen Biotech Co., Ltd. is a researcher, developer, manufacturer and marketer of more than 400 molecular, protein and cell biology products and kits for the life science research and diagnostics.
In 2001, our company was founded by three scientists, who have more than 40 years' combined life science research experience and more than 50 publications, with a mission to produce innovative and cost-effective products for the life science research. On March 21, 2006, TransGen Biotech was incorporated in Beijing, China. Our company's headquarters and manufacturing facilities are located in Beijing.
To date, our company has employed more than 100 employees in Beijing, and has more than 30 distribution centers covering almost all major cities in China. Our rich R&D experience and state-of-the-art facilities enable us to keep generating the most innovated and the highest quality products. From 2006 and 2009, our company was consecutively awarded as "High Tech Corporation in Beijing" by Beijing Local Government.
Currently, our products cover: Plasmid based DNA markers, high efficiency chemically competent cells, 5-minute PCR product cloning, a variety of PCR enzymes, RNase H deficient and high-temperature RT enzymes, one-step and two-steps qPCR enzymes, the highest efficiency mutagenesis kits, high quality nucleic acid extraction and purification kits, 5-minute expression vectors, unstained and prestained protein markers, Western Blot markers, and transfection reagents.
As a leading bioreagent company in China, we are looking forward to partnerships with you in your quest for ground-breaking life science discoveries.
