Product Specifications
| Prod Model: |
710102 |
| Markets: |
North America,South America,Eastern Europe,Southeast Asia,Africa,Oceania,Mid East,Eastern Asia,Western Europe |
Product Description
1. Summary
This kit use NDV solid phase antigen to detect specific antibody against NDV antigen, have high sensitivity, specificity, reproducibility, and is easy to operate quickly and can be used dividually in multiple times
2. Reagents and contents
1. 8× Coated microtiter strips: Ready to use
2. 1× Concentrated Conjugate solution: Ready to use
3. 1× Washing solution: Ready to use
4. 1× Substrate A solution: Ready to use
5. 1× Substrate B solution: Ready to use
6. 1× Sample dilution: Ready to use
7. 1× Stopping solution: Ready to use
Warning: Stopping solution irritates eyes and skin. Keep out of the reach of children. Upon contact with the eyes, rinse thoroughly with water and consult a doctor.
8. 1× Positive control: Ready to use
9. 1× Negative control: Ready to use
10. 1× Instruction sheet
3. Test procedure
1. Diluting concentrated washing solution
Dilute the concentrated washing solution with distilled water or deionized water at 1: 10, e. G. Add 270 ml distilled water in 30 ml concentrated washing solution, and mix thoroughly.
2. Sample preparation:
Dilute the serum sample with sample dilution at 1: 100, e. G. 5 μl serum + 495 μl sample dilution, mix thoroughly.
3. Adding samples and controls
Leave well A1 as reagent blank. Pipette controls and samples as follows:
100 μl of negative control and positive control respectively, and 100 μl diluted samples each into remaining wells.
Incubation at 37 degrees Celsius for 30 minutes. Discard liquid of the wells and fill all wells with diluted washing solution, incubate for 1 minute and discard. Repeat washing procedure two more times as above. At the end of the washing step, carefully remove remaining fluid by tapping the strips on the tissue paper prior to the next step.
4. Adding conjugate solution
Add 1 drop conjugate solution into all wells and incubate at 37 degrees Celsius for 30 minutes. Discard liquid of the wells and wash 3 times as described in step 3.
5. Adding substrates
Add one drop of substrate A solution and B solution respectively, incubate at 37 degrees Celsius for 10 minutes.
Add one drop of stopping solution into all wells. Before reading results with ELISA microtiter plate reader, set the reagent blank well A1 to zero. Measure the absorbance of all wells at 450 nm (reference wavelength: 620 nm).
Shenzhen Finder Biotechnology Co., Ltd. (hereinafter referred to as the Finder Biotechnology ) is located in the scenic Shenzhen Baoan Shi Yanhong High-tech Park, is a professional veterinary drugs, food additives, hormones, mycotoxins and other residues rapid detection of animalepidemics of rapid diagnostic technology research and product development of the national high-tech enterprises.
Finder Biotechnology set biotechnology research, production, marketing and technical services four one, based on food safety to develop the fastest, most effective detection products, independent research and development of veterinary drug residue rapid test kits and rapid test strip card and continuously meet thethe needs of the domestic food safety testing, product up to dozens of technical standards and operational efficiency far more than in foreign countries; Followed by livestock and poultry-based animal disease diagnostic kits and test strip cards are widely used in animal husbandry units, researchunits and institutions of higher learning, to monitor immune and diagnostic effect provide a strong basis and safeguards; Fender bio-play innovation capability and professional capacity, advancing with the times, the relevant departments of food safety monitoring and food production enterprisesregulators to provide effective assistance and protection.
Finder Biotechnology will create international food safety testing technology platform for the detection of domestic and foreign customers to test standard and technical exchanges. Adhering to the "to provide more quality products, and more quality services, the pursuit of quality and win-win" mission, and constantly improve the efficient customer service system, give full play to the advantage of technology in the field of food safety testing and continuous ability to innovate, to become the international bio-detection reagent most the biological corporate leadership.
