Product Specifications
| Prod Model: |
ES1071 |
| Markets: |
North America,South America,Eastern Europe,Southeast Asia,Africa,Mid East,Eastern Asia,Western Europe |
Product Description
Proteinase K (20mg/ml, Solution)
Cat# ES1071 1ml
Grade: PCR grade, for Molecular Biology
Storage Temperature -20° C
Description
Proteinase K is an endolytic protease that cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic or hydrophobic amino acids. The Proteinase K is classified as a serine protease. The smallest peptide to be hydrolyzed by this enzyme is a tetrapeptide. The recommended working concentration for Proteinase K is 0.05-1 mg/ml.
Applications
< 1> for isolation of genomic DNA from different kind of samples.
< 2> for isolation of total RNA from different kind of samples.
Inhibition and Inactivation
Inhibitors: Proteinase K is not inactivated by metal chelators, by thiol-reactive reagents or by specific trypsin and chymotrypsin inhibitors. Phenylmethylsulfonyl fluoride and diisopropyl phosphorofluoridate completely inhibit the enzyme.
Inactivated by heating at 95° C for 10 minutes.
Note:
Optimum activity at 50-55° C.
Rapid denaturation of enzyme occurs at temperatures above 65° C.
The recommended working concentration for Proteinase K is 0.05-1 mg/ml. The activity of the enzyme is stimulated by 0.2-1% SDS or by 1-4 M urea.
Ca2+protects Proteinase K against autolysis, increases the thermal stability and has a regulatory function for the substrate binding site of Proteinase K.
Stable over a wide pH range: 4.0-12.5, optimum pH 7.5-8.0.
Guangzhou Geneshun Biotech Ltd., is mainly engaged in developing, manufacturing and marketing of life science product and devotes to provide innovative product for life science researchers. Through advanced technologies, we offer many different innovative kits and reagents and one-to-one support to help researchers reach their goals faster with greater success.
A series of products with high quality and high stability have been developed since the company's establishment. Gene Shun Biotech provide a wide variety of popular products, including Nucleic Acid Isolation&PurificationHigh pure Plasmid DNA Purification kits; PCR products/Agarose Gel DNA Purification Kits; Genomic DNA purification kits), DNA Molecular Weight Markers(50bp DNA ladder; 100bp DNA ladder; 1kb DNA ladder), PCR-related Products(Taq DNA Polymerase; High Taq DNA polymerase, Pfu DNA Polymerase; PCR Taq Mix; dNTP mix; dNTP sets), RT-PCR Products(Super M-MuLV Reverse Transcriptase; H Minus M-MuLV Reverse Transcriptase; First Strand cDNA Synthesis Kit); RNA-related Products, Molecular biology reagent(Agarose, Proteinase K, Ribonuclease A) and so on.
GeneShun is ISO 9001: 2008 certified, assuring quality and services. GeneShun is committed to bringing the highest quality life science reagents to our customers. The outstanding quality, stable quality and reasonable price help GeneShun Biotech Ltd gain the general scientific researcher's fancy and our product becomes one of the best choice to many researchers at home and abroad. Many products have been exported to oversea, such as USA, Germany, Australia, Israel, Russia, India, Pakistan, Japan and Koreas.
