Product Specifications
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North America,South America,Eastern Europe,Southeast Asia,Africa,Oceania,Mid East,Eastern Asia,Western Europe |
Product Description
2x PCR PreMix, with dye
The PCR master mix simplifies the assembly of PCR reaction and offers advantages of time savings, convenience, consistency, and minimal risk of contamination and pipetting errors.
The tracking dye and precipitant have been added into the PreMix so that the PCR product can be directly loaded for electrophoresis.
Features of the Taq DNA polymerases contained in the PCR master mix:
# High efficiency. The extension time of the Taq DNA polymerase is shorter than 30 seconds per kb DNA. Easily amplify DNA fragments up to 4 kb.
# High purity. >98% homogeneous of the Taq DNA polymerase by SDS gel electrophoresis.
# Reproducible results. No visible activity change after storage of the Taq DNA polymerase at room temperature for 1 months to amplify a single-copy gene from human genome with high efficiency.
# Terminal transferase activity of adding a single nucleotide (adenosine) at 3' end of the extension product, facilitating TA cloning of PCR products.
Description:
The 2x PCR PreMix with non-interfering dye is a ready-to-use 2x concentrated solution. Except DNA template and primers, the PCR master mix contains all components for PCR, including Taq DNA polymerase, dNTPs, MgCl2, reaction buffer, PCR stabilizer and enhancer at optimal concentrations for consistent and efficient PCR amplification. For a 20 µ L PCR reaction, simply add 10 µ L of 2x PCR PreMix to specific primers, DNA template, and water.
Optimal template and primer concentrations, and PCR program parameters should be determined experimentally by the investigator.
Applications of the PCR master mix:
# Routine PCR using genomic, viral and plasmid templates.
# RT-PCR.
# DNA cloning and subcloning.
# Primer extension.
# DNA and colony screening.
# Genotyping.
# DNA mutagenesis.
# Generating hybridization probes for Southern or Northern hybridization.
The Taq DNA polymerase contained in the 2X PCR PreMix, with dye is available alone.
Storage/Stability: Stable for 1 year at -20° C and 3 months at 4° C. For maximum recovery of the product, centrifuge the original vial after thawing and before removing the cap. Aliquot to avoid repeated freezing and thawing.
Aidlab Biotechnologies Co., Ltd was founded by Returnee Overseas Students in 2009. The company is located in Tsinghua Science Park@Zicheng Pioneer Park in Beijing, the most famous Chinese High-Tech area. Our team comprises of a large group of experienced scientists and promising marketing specialists.
Main products include extraction and purification products of nucleic acid; PCR and RT-PCR; Real time PCR, DNA marker; T vector and cloning kits; Topoisomerase based TA or Blunt cloning vector; Protein reagents; Protein expression and purification for medical or research use. Based on consistent R & D investement, we established a standardized manufacturing program and a comprehensive quality assurance model for large-sale production of reagents for life science. We guarantee to provide a series of products with stable performance and high-quality for research worldwide.
Our platforms and services:
Extraction of nucleic acids from general and tough samples, including human, animals, plants, microbes, soil, stool, etc.
PCR, RT-PCR and Real-time PCR
T vector and cloning service
Western blot
Large scale production of endotoxin free plasmids or general plasmids
Expression, fermentation, purification of proteins for research use or medical use
Sequencing service
Customer oligos
