Product Specifications
Usage: |
Positive Catalyst |
Classification: |
Biocatalysts |
Product Description
Reliable quality and low price. Low to 25$/10000 Units( 50 applications)
M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant:
Description: Moloney Murine Leukemia Virus Reverse Transcriptase, RNase H Minus, Point Mutant (M-MLV RT (H minus) is an RNA-dependent DNA polymerase that can be used in cDNA synthesis with long RNA templates (> 5kb). The lack of RNase H activity is germane to this application in that this activity can start to degrade template when incubation times are long, as they may need to be when making long cDNAs. Although many researchers are successful in using M-MLV RT (H+) for analytical and some preparative cDNA applications, reverse transcriptases lacking RNase H activity provide another option for the preparation of long cDNAs and libraries containing a high percentage of full-length cDNA.
An application of M-MLV RT (H minus) is first-strand synthesis of cDNA from RNA molecules.
Enzyme Storage Buffer: M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant is supplied in 20mM Tris-HCl (pH 7.5), 200mM NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.
M-MLV Reverse Transcriptase 5X Reaction Buffer: When the M-MLV Reverse Transcriptase 5X Reaction
Buffer supplied with this enzyme is diluted 1: 5, it has a composition of 50mM Tris-HCl (pH 8.3), 75mM KCl, 3mM MgCl2 and 10mM DTT.
Source: Purified from an E. Coli strain expressing a recombinant enzyme
Storage Temperature: Store at minus 20.
PC3301 | 2 x SYBR Green qPCR Mix | 1.25ml | 25 | PC3302 | 5ml | 80 | PC38 | Rox Reference Dye( 50μM) | 5ml | 100 |
| N6 Random primer | 25 OD | 50 |
| Oligo dT18 | 25 OD | 30 | PC1702 | TUREscript H Minus M-MuLV Reverse Transcriptase | 10000U | 30 | PC1703 | 50000U | 125 | PC1802 | TUREscript 1st Strand cDNA Synthesis Kit | 50 reactions | 50 | PC4301 | THERMOscript H Minus M-MuLV Reverse Transcriptase | 10000U | 60 | PC4302 | 50000U | 250 | PC4402 | THERMOscript 1st Strand cDNA Synthesis Kit | 50 reactions | 75 | PC3401 | TUREscript One Step RT-PCR Kit | 50 reactions | 40 | PC3402 | 100 reactions | 80 | PC3602 | TUREscript SYBR Green qRT-PCR Kit | 50 reactions | 60 |
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Aidlab Biotechnologies Co., Ltd was founded by Returnee Overseas Students in 2009. The company is located in Tsinghua Science Park@Zicheng Pioneer Park in Beijing, the most famous Chinese High-Tech area. Our team comprises of a large group of experienced scientists and promising marketing specialists.
Main products include extraction and purification products of nucleic acid; PCR and RT-PCR; Real time PCR, DNA marker; T vector and cloning kits; Topoisomerase based TA or Blunt cloning vector; Protein reagents; Protein expression and purification for medical or research use. Based on consistent R & D investement, we established a standardized manufacturing program and a comprehensive quality assurance model for large-sale production of reagents for life science. We guarantee to provide a series of products with stable performance and high-quality for research worldwide.
Our platforms and services:
Extraction of nucleic acids from general and tough samples, including human, animals, plants, microbes, soil, stool, etc.
PCR, RT-PCR and Real-time PCR
T vector and cloning service
Western blot
Large scale production of endotoxin free plasmids or general plasmids
Expression, fermentation, purification of proteins for research use or medical use
Sequencing service
Customer oligos