Product Specifications
| Prod Model: |
710102 |
| Markets: |
North America,South America,Eastern Europe,Southeast Asia,Africa,Oceania,Mid East,Eastern Asia,Western Europe |
Product Description
1. Usage
The porcine encephalitis virus ELISA test kit is used for the detection of the porcine encephalitis virus antibodies in swine serum; Assessment the immunity conditions against porcine encephalitis virus in the pig farms and investigation of the epidemiology of the porcine encephalitis virus.
2. Principle
The kit is based on an indirect enzymatic immunoassay(Indirect ELISA). The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific peroxidase conjugate(IgG-HRP). After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer(630 nm).
3. The kit components
1 Antigen coated microtiter plate 2 pieces (96 wells)
2 Negative (green lid)/positive (red lid)serum 1 tube each (1 mL/tube)
3 Anti-pig IgG-HRP conjugate (yellow lid) 1 bottle (22 mL/bottle)
4 20× Concentrated washing buffer (white lid) 1 bottle (30 mL/bottle)
5 Substrate A (purple lid)/B (black lid) 1 bottle each (12 mL/bottle)
6 Stop solution(blue lid) 1 bottle (12 mL/bottle)
7 Sample diluent solution(transparent lid) 1 bottle (50 mL/bottle)
4. Precautions and warnings for users
1. Read the use instructions carefully.
2. Bring all reagents to room temperature prior to use.
3. Do not use components after expiration dates and do not mix components from different lots.
4. If the dry bag is red, don't use.
5. Don't pollute any components.
6. Reseal the plates after use and avoid to wet.
7. Do not pipette by mouth
8. Use gloves and Be careful with reagents
9. Clean the discarded reagents
5. Materials Required But Not Provided
Micropipettes, Distilled water, Incubator, Reservoir, Spectrophotometer, Absorbent paper.
6. Preparation
Washing buffer: Dilute 20× Concentrated washing buffer with deionized water at 1: 19.
7. Test Procedure
1. Take out the coated plates(Can be detached) and record the sample position on a worksheet. Set 2 wells for negative control serum and 2 wells for positive control serum, 100 μL/well. Set one blank control well, add 100 μL sample diluent. Similarly, dilute the samples at 1: 39 with the sample diluent, 100 μL each well. Mix gently, incubate at 37degrees Celsius for 30 min.
2. Wash each well with 300 μL washing buffer 3 times, and each static for 3 min. The last time, pat to dry on absorbent paper.
3. Add 100 μL enzyme comjugate into each well, and incubate at 37degrees Celsius for 30 min.
4. Repeat step 2.
5. Add 50 μL substrate A and 50 μL substrate B into each well, mix properly, incubate for 10 min at room temperature in the dark.
6. Add stop solution one drop (50 μL) to each well, and determine the result within 10 min.
Shenzhen Finder Biotechnology Co., Ltd. (hereinafter referred to as the Finder Biotechnology ) is located in the scenic Shenzhen Baoan Shi Yanhong High-tech Park, is a professional veterinary drugs, food additives, hormones, mycotoxins and other residues rapid detection of animalepidemics of rapid diagnostic technology research and product development of the national high-tech enterprises.
Finder Biotechnology set biotechnology research, production, marketing and technical services four one, based on food safety to develop the fastest, most effective detection products, independent research and development of veterinary drug residue rapid test kits and rapid test strip card and continuously meet thethe needs of the domestic food safety testing, product up to dozens of technical standards and operational efficiency far more than in foreign countries; Followed by livestock and poultry-based animal disease diagnostic kits and test strip cards are widely used in animal husbandry units, researchunits and institutions of higher learning, to monitor immune and diagnostic effect provide a strong basis and safeguards; Fender bio-play innovation capability and professional capacity, advancing with the times, the relevant departments of food safety monitoring and food production enterprisesregulators to provide effective assistance and protection.
Finder Biotechnology will create international food safety testing technology platform for the detection of domestic and foreign customers to test standard and technical exchanges. Adhering to the "to provide more quality products, and more quality services, the pursuit of quality and win-win" mission, and constantly improve the efficient customer service system, give full play to the advantage of technology in the field of food safety testing and continuous ability to innovate, to become the international bio-detection reagent most the biological corporate leadership.
