Quinolone Rapid Test Kit (Aquatic Products Test Kit)

Product Specifications

Prod Model:	W81074-3
Markets:	North America,South America,Eastern Europe,Southeast Asia,Africa,Oceania,Mid East,Eastern Asia,Western Europe
Principle:	Lateral Flow Immunoassay
Sensitivity:	20 Ppb
Specificity:	98%
Specimen:	Aquatic Products
Assay Time:	5 - 10 Min
Shelf Life:	18 Months
Storage:	2 - 30 C
Certificate:	ISO 9001 & 13485
Options:	OEM Available

Product Description

Quinolone Rapid Test (Aquatic Products)
Cat No.: W81074-3

1. INTENDED USE
Quicking Quinolone Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Quinolone residue in aquatic product sample.
Cut-off: 20 ppb
Assay Time: 5 - 10 min

2. PRINCIPLE OF THE ASSAY
Quicking Quinolone Rapid Test is based on competitive lateral flow immunochromatographic assay. The Quinolone conjugate in the test zone will capture the immuno-gold (colloid gold-Quinolone antibody conjugate), when there is very little dissociative Quinolone in the samples. A visible red test band indicates a negative result when the control line (C zone) shows that the card is valid. The test band (T zone) will be not visible if Quinolone is present in concentration of 20 ppb and above which explains a positive result.

3. KIT COMPONENT
- 10× Foil pouches each containing one cassette and a desiccant
- 1× Plastic canister containing 10 microwells and a desiccant
- 2× Assay buffer (Diluent A, 30 mL, Diluent B, 8 mL)
- 20× Pipettes, 2× Centrifugal tubes (50 mL)
- Products Manual

4. TEST PROCEDURE
- Get rid of the fat tissue and cut down the sample materials. Homogenize the sample at 10000 rpm for 1 min. Weigh out 4.0 g of sample into a 50 mL centrifugal tube, add 2 mL of Diluent A and then tightly cover the lid. Shake the tube sufficiently.
- Add 3 mL of Ethyl Acetate and 6 mL of Methylene chloride, tighten the lid and shake for 5 min. Stand the tube until the organic phase at under layer gets clear.
- Transfer 5 mL of organic phase into a 10 mL of beaker. Dry the liquid by blowing wind. Redissolve the residue in the beaker with 0.3 mL of Diluent B.
- Take out the microwells strip from the plastic canister. Take one well and tear off the film. Transfer 0.2 mL (reticle on the pipette) of the underlayer liquid into the well. Repeatedly suck and extrude the sample until all red reagents are completely dissolved. Wait for 1 min.
- Take out the cassette from the foil pouch and place it horizontally.
- Suck the mixture in the well and gradually drip 3 drops into the assay sample hole "S".
- Interpret the result in 5-10 min. Result after 10 min is considered as invalid.

5. INTERPRETATION OF RESULTS
Positive: Only one clear band in C zone indicates a positive result. Positive shows that the concentration of Quinolone is at or above 20 ppb in the samples.
Negative: The presence of both clear bands in C zone and T zone.
Invalid: No colored band appears in C zone.

6. SPECIFICITY
The results are negative when the test card is applied to detect 100 ppm (μ G/mL) of Chloramphenicol, Aminoglycosides, Beta-lactams, Sulfonamides, Tetracyclines and Macrolides.

7. STORAGE
The kit can be stored at room temperature (2-30° C). The test kit is stable through the expiration date (12 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

8. PRECAUTIONS
- For best results, please strictly adhere to these instructions.
- All reagents must be at room temperature before running the assay.
- Do not remove test cassette from its pouch until immediately before use.
- The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

9. LIMITATION
Quicking Quinolone Rapid Test is an useful tool offering a rapid and accurate testing in field detection, exceeding with its convenience. It provides a semi-quantitative method to detect the existing of Quinolone above 20 ppb in aquatic product samples. If you want a quantitative result, please adopt other method such as ELISA/ HPLC in practice.