Streptomycin Rapid Test Kit (Aquatic Products Test Kit)

Product Specifications

Prod Model:	W81028-3
Markets:	North America,South America,Eastern Europe,Southeast Asia,Africa,Oceania,Mid East,Eastern Asia,Western Europe
Principle:	Lateral Flow Immunoassay
Sensitivity:	100 Ppb
Specificity:	98%
Specimen:	Aquatic Products
Assay Time:	5 - 10 Min
Shelf Life:	18 Months
Storage:	2 - 30 C
Certificate:	ISO 9001 & 13485
Options:	OEM Available

Product Description

Streptomycin Rapid Test (Aquatic Products)
Cat No.: W81028-3

1. INTENDED USE
Quicking Streptomycin Rapid Test is a competitive immunoassay for the semi-quantitative detection of the presence of Streptomycin residue in aquatic product sample.
Cut-off: 100 ppb
Assay Time: 3 - 5 min

2. PRINCIPLE OF THE ASSAY
Quicking Streptomycin Rapid Test is based on competitive lateral flow immunochromatographic assay. The Streptomycin conjugate in the test zone will capture the immuno-gold (colloid gold-Streptomycin antibody conjugate), when there is very little dissociative Streptomycin in the samples. A visible red test band indicates a negative result when the control line (C zone) shows that the card is valid. The test band (T zone) will be not visible if Streptomycin is present in concentration of 100 ppb and above which explains a positive result.

3. KIT COMPONENT
- 10× Foil pouches each containing one cassette and a desiccant
- 1× Plastic canister containing 10 microwells and a desiccant
- 10× Pipettes
- 2× Centrifugal tubes (5 mL)
- Product Manual

4. TEST PROCEDURE
Materials selection. Fish: Get rid of the squama and skin, collect muscle along the fish spine. Shrimp: Get rid of the head, shell and intestinal gland, collect the muscle tissue as sample materials. Crab and turtle, take the edible parts as sample materials.
- Get rid of the fat tissue and cut down the sample materials. Homogenize the sample at 10000 rpm for 1 min. Weigh out 2 g of the homogenized sample and place into a 5 mL centrifugal tube.
- Add 1 mL of assay buffer and put the tube on Vortex for 5 min.
- Do a centrifugation at 4000 rpm for 2-3 min.
- Take out the microwells strip from the plastic canister. Take one well and tear off the film. Transfer 0.2 mL (reticle on the pipette) of the underlayer liquid into the well. Repeatedly suck and extrude the sample until all red reagents are completely dissolved. Wait for 1 min.
- Take out the cassette from the foil pouch and place it horizontally.
- Suck the mixture in the well and gradually drip 3 drops into the assay sample hole "S".
- Interpret the result in 3 - 5 min. Result after 5 min is considered as invalid.

5. INTERPRETATION OF RESULTS
Positive: Only one clear band in C zone indicates a positive result. Positive shows that the concentration of Stretomycin is at or above 100 ppb in the samples.
Negative: The presence of both clear bands in C zone and T zone.
Invalid: No colored band appears in C zone.

6. SPECIFICITY
The results are negative when the test card is applied to detect 100 ppm (μ G/mL) of Chloramphenicol, Aminoglycosides, Beta-lactams, and Fluoro quinolones.

7. STORAGE
The kit can be stored at room temperature (2-30° C). The test kit is stable through the expiration date (12 months) marked on the foil pouch. DO NOT FREEZE. Do not store the test kit in direct sunlight.

8. PRECAUTIONS
- For best results, please strictly adhere to these instructions.
- All reagents must be at room temperature before running the assay.
- Do not remove test cassette from its pouch until immediately before use.
- Do not use the test beyond its expiration date marked on the foil pouch.
- The components in this kit have been quality control tested as standard batch unit. Do not mix components from different lot numbers.

9. LIMITATION
Quicking Streptomycin Rapid Test is an useful tool offering a rapid and accurate testing in field detection, exceeding with its convenience. It provides a semi-quantitative method to detect the existing of Streptomycin above 100 ppb in aquatic product samples. If you want a quantitative result, please adopt other method such as ELISA/ HPLC in practice.